2H-Cho-Arg (trifluoroacetate salt) - CAS 1609010-59-4

2H-Cho-Arg is a steroid-based cationic lipid that contains a 2H-cholesterol skeleton coupled to an L-arginine head group and can be used to facilitate gene transfection. It forms a complex with plasmid DNA (pDNA) and decreases pDNA migration in an electrophoretic mobility shift assay at +/- charge ratios of 4 or higher. 2H-Cho-Arg facilitates transfection of a luciferase gene into H1299 cells, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein , but not by inhibitors of clathrin- or micropinocytosis-mediated endocytosis. It induces cytotoxicity in H1299 cells (IC50 = 92.7 μg/ml) .

Others, Liposome

Others, Metabolism

1608947

C43H73F6N5O7

886.06

OC(C(F)(F)F)=O.C[C@@]12[C@]3([H])[C@](CC[C@@]1([H])C[C@H](CC2)OC(CCCCCNC([C@@H](N)CCCNC(N)=N)=O)=O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCCC(C)C)C.OC(C(F)(F)F)=O

-20°C

Ice Packs

Small Compound