3,6-diacetoxy Phthalonitrile - CAS 83619-73-2

3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.

Others

Others

83544

C12H8N2O4

244.206

CC(=O)Oc1ccc(OC(C)=O)c(C#N)c1C#N

-20°C

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Dye-Reagents