4-Methylumbelliferyl β-D-N, N'-diacetylchitobioside - CAS 53643-12-2

4-Methylumbelliferyl β-D-N, N'-diacetylchitobioside (4-μU- (GlcNAc) 2) is a fluorogenic substrate for chitinases and chitobiosidases. 4-μU- (GlcNAc) 2 is cleaved by chitinases and chitobiosidases to release the fluorescent moiety 4-μU. 4-μU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases.

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53629

C26H34N2O13

582.55

CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](Oc2ccc3c(C)cc(=O)oc3c2)[C@H](NC(C)=O)[C@H]1O

-20°C

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