5-Fluoroorotic acid monohydrate - CAS 220141-70-8

5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono (hydrate) ; 5-FOA) is employed to detect the expression of the URA3 gene in yeast molecular genetic constructs. Yeast possessing an active URA3 gene (which encodes orotidine-5'-monophosphate decarboxylase) convert 5-FOA into 5-fluorodeoxyuridine, a substance toxic to cells. Yeast strains with mutations in the URA3 gene that confer resistance can grow in the presence of 5-FOA if uracil is added to the medium.

Others

Others

220063

C5H5FN2O5

192.1

O=C1NC(=O)C(F)=C(N1)C(=O)O.O

-20°C

Ice Packs

Small Compound