Dios-Arg (trifluoroacetate salt) - CAS 1807353-31-6

Dios-Arg is a steroid-based cationic lipid that contains a diosgenin skeleton coupled to an L-arginine head group. It forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio greater than or equal to 4. Dios-Arg facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence and absence of fetal bovine serum, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin and the caveolae-mediated endocytosis inhibitor genistein . It has been used, coupled to 1,2-dioleoyl-sn-glycero-3-PE , to bind siRNA and plasmid DNA to form cationic lipid nanoparticles for intracellular transport. Dios-Arg is cytotoxic to H1299 and HeLa cells (IC50s = 83.5 and 74.1 μg/ml, respectively) .

Others, Liposome

Metabolism, Others

1807316

C43H67F6N5O9

912.01

OC(C(F)(F)F)=O.C[C@@]12[C@]3([H])[C@](O[C@]4(CC[C@H](CO4)C)[C@H]3C)([H])C[C@@]1([H])[C@@]5([H])[C@]([C@@]6(C(C[C@H](CC6)OC(CCCCCNC([C@@H](N)CCCNC(N)=N)=O)=O)=CC5)C)([H])CC2.OC(C(F)(F)F)=O

-20°C

Ice Packs

Small Compound